Abstract
A new acetylenic acid, (10E,14Z)-9-oxooctadeca-10,14-dien-12-ynoic acid (1), was isolated from the edible mushroom Chanterelle (Cantharellus cibarius), together with a known acetylenic acid, (10E,14Z)-9-hydroxyoctadeca-10,14-dien-12-ynoic acid (2) and their structures were determined through analysis of NMR and mass data. The new acetylenic acid (1) specifically activated peroxisome proliferator-activated receptor (PPAR)-γ with an EC(50) value of 1.88 μM as measured by a reporter gene assay. Expression of PPAR-γ target genes were significantly altered as well, supporting the hypothesis that compound 1 is a PPAR-γ potential agonist that regulates transcription of the PPAR-γ target genes.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agaricales / chemistry*
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Alkynes / chemistry*
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Alkynes / isolation & purification
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Alkynes / pharmacology
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Animals
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / pharmacology
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CHO Cells
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Chromans / pharmacology
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Cricetinae
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Dose-Response Relationship, Drug
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Fatty Acids, Unsaturated / chemistry*
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Fatty Acids, Unsaturated / isolation & purification
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Fatty Acids, Unsaturated / pharmacology
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Gene Expression
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Genes, Reporter
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Hep G2 Cells
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Humans
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Hypoglycemic Agents / chemistry*
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Hypoglycemic Agents / isolation & purification
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Hypoglycemic Agents / pharmacology
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Luciferases / genetics
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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PPAR gamma / agonists*
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PPAR gamma / genetics
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PPAR gamma / metabolism
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Thiazolidinediones / pharmacology
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Transfection
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Troglitazone
Substances
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Alkynes
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Antineoplastic Agents
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Chromans
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Fatty Acids, Unsaturated
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Hypoglycemic Agents
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PPAR gamma
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Thiazolidinediones
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2-hexadecynoic acid
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Luciferases
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Troglitazone